R&D: Photonic Microspheres for High-Capacity DNA Data Storage, Robust, Straightforward, and Scalable Random Access via Nonfading Indexes

Science Advances has published an article written by Qiu-Jun Liu, Tianjin Key Laboratory of Composite and Functional Materials, School of Materials Science and Engineering, Tianjin University, Tianjin 300350, China, Qian Liu, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China, Jie Zhang, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China, Cheng Zhang,School of Computer Science, Key Laboratory of High Confidence Software Technologies, Peking University, Beijing, China, Long Qian, Center for Quantitative Biology, Peking University, Beijing, China, Hao Qi, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China, and State Key Laboratory of Synthetic Biology, Tianjin University, Tianjin 300072, China, Yue-Sheng Li, Tianjin Key Laboratory of Composite and Functional Materials, School of Materials Science and Engineering, Tianjin University, Tianjin 300350, China, and Dong-Po Song, Tianjin Key Laboratory of Composite and Functional Materials, School of Materials Science and Engineering, Tianjin University, Tianjin 300350, China, and State Key Laboratory of Synthetic Biology, Tianjin University, Tianjin 300072, China.

Abstract: “DNA-based storage offers a compelling alternative to traditional optical and magnetic devices. However, random data access usually requires additional noncoding primer DNA as indexes, which substantially reduce the physical data density. Here, we propose an alternative strategy to overcome this barrier by loading different data-encoding DNA files into porous microspheres, each distinguished by unique photonic bandgaps and diameters, allowing for 10⁵ types of indexing. With the two features as the addressing indexes, the physical separation of subsets from a diverse pool of DNA files is achieved, thereby facilitating the selective retrieval of stored data. The interconnected nanopore arrays and uniformly distributed positive charges within the microspheres enhance DNA enrichment, achieving a storage density of up to 22.6 exabytes per gram, far exceeding that of previous random access storage methods. This work outlines a simple and scalable method for creating nonfading photonic indexes, enabling long-term random access while maintaining high storage capacity.“