Chinese Researchers Make DNA Storage Breakthroughs

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Chinese researchers make DNA storage breakthroughs
Chinese researchers have recently made technical breakthroughs in DNA storage, achieving the integration of DNA synthesis and sequencing and miniaturization of instruments.The research team from the Southeast University in Nanjing, East China’s Jiangsu province, has successfully encoded the motto “Strive for Perfection” into a piece of DNA sequence and also successfully read it out.

Resource:
Article: Electrochemical DNA synthesis and sequencing on a single electrode with scalability for integrated data storage

Science Advances has published an article written by Chengtao Xu, Biao Ma, Zhongli Gao, Xing Dong, Chao Zhao, and Hong Liu, State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering Southeast University, 2# Sipailou, Nanjing, Jiangsu 210096, China.

Abstract: “DNA has been considered as a compelling candidate for digital data storage due to advantages such as high coding density, long retention time, and low energy consumption. Despite many works reported, the development of a DNA-based database of full integration, high efficiency, and practical applicability is still challenging. In this work, we report the synthesis and sequencing of DNA on a single electrode with scalability for an integrated DNA-based data storage system. The synthesis of DNA is based on phosphoramidite chemistry and electrochemical deprotection. The sequencing relies on charge redistribution originated from polymerase-catalyzed primer extension, leading to a measurable current spike. By regeneration of the electrode after sequencing, repeated sequencing can be achieved to improve the accuracy. A SlipChip device is developed to simplify the liquid introduction involved in DNA synthesis and sequencing. As the proof-of-concept experiment, text information is stored in the system and then accurately retrieved.“